Disease Models of Pharmacology in Preclinical CRO

• METABOLIC DISEASE MODELS

  Indication	Model	Species
  Obesity	Diet-induced Obesity (DIO)	Mice, cynomolgus
  	Ob/ob mice
  Type 1 diabetes mellitus	STZ-induced T1DM	Mice, rats, cynomolgus
  Type 2 diabetes mellitus	db/db mice	db/db mice
  	ZDF rat	ZDF rat
  	STZ + HFD-induced T2DM	mice
  	Spontaneous T2DM	cynomolgus
  	Euglycaemic clamp	Beagle dog
  Hyperlipidemia/ Hypercholesterolemia	HFHC diet	ApoE mice, Golden hamster, cynomolgus
  Atherosclerosis	HFHC diet	ApoE mice
  	HFHC diet + mechanical vessel injury	Rabbits, cynomolgus
  Hyperuricemia	Spontaneous hyperuricemia	UOX mice

• DIABETES COMPLICATIONS

  Indication	Model	Species
  Diabetic nephropathy	STZ + nephrectomy + high salt load	rat, mice
  Delayed diabetic wound healing	STZ-induced T1DM + skin excision	rat
  	T2DM + skin excision	db/db mice, ZDF rat
  Diabetic neuralgia	STZ-induced T1DM	mice

• SKIN

  Indication	Model	Species
  Wound healing	Skin excision	rat, mice, swine
  Burn	Burn	swine

• CNS - CENTRAL & PERIPHERAL NERVOUS SYSTEM DISEASES

  Indication	Model	Species
  Alzheimer's disease	APP/PS1 Mice	mice
  	5XFAD Mice	mice
  Parkinson's disease	6-OHDA-induced PD	rat
  	MPTP-induced PD	monkey
  Depression/Anxiety	Forced swimming	rat, mice
  	Dangling tail	mice
  	Open field	rat, mice
  	Rlevated pluWs maze,EPM	rat, mice
  	m-CPP-induced anxiety-like symptoms	rat
  	Chronic unpredictable mild stress (CUMS)	mice
  	Learned helplessness	rat, mice
  Epilepsy	PTZ-induced acute and chronic attacks	rat, monkey
  Sleep/Sedation/Anesthesia Induction	Loss of righting reflex,LORR	rat, mice
  	Spontaneous activity level	rat, mice
  	Sedation rating	dog, swine, monkey
  	EEG telemetry	rat, mice, monkey
  Pain	acute gouty arthritis	rat, mice, UOX mice
  	Adjuvant arthritis	rat, mice
  	CIA arthritis	rat, mice, monkey
  	Carrageenan-induced plantar swelling	rat
  	Formalin causedkey pain	mice
  	Acetic acid writhing	mice
  	Nerve block	rat
  	Postoperative incision pain	rat, dog
  	Chronic neuralgia (CCI, SNI, SNL)	rat
  	Diabetic nerve pain	mice
  	Cancer pain	mice
  	Chemotherapy pain	rat
  	Trigeminal neuralgia	rat
  	MIA osteoarthritis	rat
  	ACLT Osteoarthritis	dog
  Stroke	Permanent ischemic MCAO	rat
  	Ischemia-reperfusion MCAO	rat, monkey
  Spinal Cord Injury (SCI)	Spinal cord hemisection	rat, monkey
  	Spinal cord blow injury	rat, monkey
  Drug dependence	Natural withdrawal	rat, mice
  	Urge withdrawal	rat, mice
  	Conditioned Place Preference, CPP	rat
  	Self-administration	rat
  	Drug identification	rat
  Migraine	Capsaicin-induced vasodilation model	rat, monkey
  Somatic neurotoxicity	Polio, herpes, measles, mumps, rubella, etc.	monkey

• NFLAMMATION & AUTOIMMUNE DISEASE

  Indication	Model	Species
  Rheumatoid arthritis	CFA induced arthritis	rat, mice
  	Collagen induced arthritis	rat, mice, monkey
  	Spontaneous	Tg197 mice, Tg1006 mice
  Systemic lupus erythematosus (SLE) model	Spontaneous	MRL/Lpr mice
  GvHD	PBMC transplant	NPG mice
  Ulcerative colitis	DNCB-induced colitis model	rat
  EAE	MOG35-55-induced EAE	B6 mice
  	PLP139-151-induced EAE	SJL mice
  psoriasis	Imiquimod induced psoriasis model	mice
  Atopic dermatitis models	M903-induced AD	mice
  	DNCB-induced AD	mice
  Sepsis	LPS-induced sepsis	mice
  	Cecal ligation and puncture (CLP)-induced sepsis	rat

• DIGESTIVE DISORDERS

  Indication	Model	Species
  Acute gastric ulcer	1. Water stress 2. Histamine induction 3. Ethanol induction 4. Pentagastrin induction 5. NSAID induction 6. Pylorus ligation	rat
  Chronic gastric ulcer	Acetic acid cauterization	rat
  Acute and chronic liver injury/fibrosis	CCl4 Induction	rat, mice
  	TAA Induction	rat
  	Bile duct ligation	rat
  Hepatic fibrosis/ decompensated cirrhosis	CCl4 + HFD + ethanol	rat
  Nonalcoholic fatty liver disease (NASH)	MCD-induced NASH	mice
  	CDAA + HFD-induced NASH	mice
  	GAN/ALMN diet-induced NASH	mice, ob/ob mice
  	STZ + HFD-induced NASH	newborn mice
  	CCl4 + HFD	rat
  	HFHC diet-induced NASH	cynomolgus
  	High Fructose Diet Induction	Golden hamster

• BLOOD DISORDERS

  Indication	Model	Species
  Coagulation disorders	Artery thrombosis	rat, rabbit, dog
  	Deep venous thrombosis	rat, rabbit
  	Arteriovenous (AV) shunts	rat, rabbit
  	Disseminated intravascular coagulation, DIC	mice
  	Pulmonary embolism	rat
  	Hemophilia	F8 KO mice
  Granulocyte/ thrombocytopenia	CD41 antibody-induced Immune thrombocytopenia (ITP)	mice
  	Chemotherapy-induced granulocytopenia	mice, monkey
  	Radiotherapy-induced granulocytopenia	mice, monkey
  Anemia	Anemia of renal disease	rat
  	Chemotherapy-induced anemia	rat, mice, monkey
  	Aplastic anemia	mice

• ENDOCRINE DISEASE

  Indication	Model	Species
  Osteoporosis	Ovariectomy-Induced Osteoporosis	rat, monkey
  	hRANKL-induced Osteoporosis	rat

• REPRODUCTIVE SYSTEM DISEASE

  Indication	Model	Species
  Asthenospermia	Busulfan-induced Asthenospermia	mice
  Primary ovarian insufficiency	VCD-induced Primary ovarian insufficiency	rat

• UROLOGIC DISEASES

  Indication	Model	Species
  Acute kidney injury	Unilateral Renal Ischemia-Reperfusion	rat
  	Cisplatin-induced AKI	rat, mice
  Chronic kidney disease/renal fibrosis	5/6 nephrectomy	rat, monkey
  	Gentamicin-induced CKD	rat
  	Adenine-induced CKD	rat
  	Unilateral ureteral obstruction (UUO)-induced CKD	rat